AN INCREASE OF EFFICIENCY OF THERAPEUTIC PREPARATIONS IN ONCOLOGY WITH HELP OF HIGH ENERGY IRRADIATION

 

M.A. Zabolotnyy¹, L.I. Aslamova¹,G.I.Solyanik², L.N.Kirkilevskaya³

 

¹Taras Scevchenko National University of Kyiv,Kiev, Ukraine

²R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine

³ Kyiv Medical Institute of the Ukrainian Association of Folk Medicine, Kyiv, Ukraine

 

Actuality of researches is conditioned by the necessity of creation of non-destructive physical methods of modification of antitumoral preparations for the increase of their therapeutic efficiency [1]

The of research objective - is the study and analysis of influence of previous irradiation of solvent on optical and therapeutic properties of solution of medicinal preparations of anthracycline and alkaloid rows for the increase of their efficiency.

The measurements used drugs doxorubicin ("Pharmacia Italia SpA" , Italy) and Conium (Weleda, Germany). The solvent used was a solution of sodium chloride in concentration 9 mg/ml, produced by LLC "Novofarm - Biosynthesis", Ukraine. 

During optical studies, the spectral dependences of absorption (A) of solutions of dasgs in non-irradiated and previously irradiated by electrons of a solvent in the infrared light range were measured. The measurements were carried out on an IR-Fourier spectrometer Bruker IFS 66. The spectra were determined in the energy range (ε) [103 - 2 * 103] cm-1. The energy of the irradiating electrons was 1.0 MeV. The values of the dose of absorbed (I) irradiation were 5, 15, 25, 40, 80, 100 kGy. Correlation analysis of Pearson was used in the comparative analysis of absorption (A (ε, I)) of solutions of antitumor drugs. The interval ε is evenly divided by 400 points.

The study of the ability of high-energy electron irradiation of a physiological solution, as a solvent of a drug, to modify the pharmacological activity of the antitumor drug doxorubicin was investigated in vitro using the line of cancer cells of the lung carcinoma of Lewis (LLC). It was recorded that LLC incubation for 24 hours in the presence of doxorubicin, dissolved in a non-irradiated saline solution, resulted in a concentration-dependent decrease in the number of living cells. This behavior is due to inhibition of proliferation of tumor cells (cytostatic action) and / or their death (cytotoxic action) caused by the antitumor drug. At the same time, doxorubicin, dissolved in an irradiated saline solution, leads to an increase in cytotoxic / cytostatic effects, which is most pronounced at low concentrations: so, the number of LLC live cells at concentrations below 3 μM decreased by an average of 20% (p <0.05) compared with the action of doxorubicin, dissolved in a non-irradiated physiological solution ..

Conclusions 1). Thus, preliminary radiation irradiation of the solvent, without substantially changing the medications (in all cases, the correlation coefficient exceeds the critical value), changes some of its properties in the oscillation range, which significantly depends on the conformational state of the molecules. 2)The ability of high-energy electron irradiation of a solvent to increase the pharmacological activity of antitumor drugs konium and doxorubicin (especially in the range of low concentrations of drugs) is established. The low variability of cell survival rates after incubation with doxorubicin in irradiated physiological saline as compared to the corresponding indices with non-irradiated solvent was registered.

 

1.V.B. Orel , M.A. Zabolotny , V.E. Orel  , Med Hypotheses 102, 82 (2017).